Nutritional evaluation of two composite linseed-based ingredients for growing rabbits
Submitted: 2023-12-04
|Accepted: 2025-01-14
|Published: 2025-03-31
Copyright (c) 2025 Davi Savietto, Carole Bannelier, Michel Colin , Mathieu Guillevic , El-Sayed M. Abdel-Kafy , Sebastiana Failla , Alessandro Dal Bosco , Bayrem Jemmali , Imène Ben Salem, Gabriele Brecchia , Thierry Gidenne
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
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Keywords:
Oryctolagus cuniculus, linseed, flaxseed, rabbit nutrition, digestibility, feed formulation
Supporting agencies:
This study received financial support from the European Union - Partnership for Research and Innovation in the Mediterranean Area. Project ID 1642 - Omega Rabbit: food for health Benefit.
Abstract:
The digestible energy and protein content of two novel linseed-based ingredients were measured in growing rabbits: a high-fat extruded linseed meal cooked (ELM) and a mixture (70:30) of high-fat linseed chaff and wheat flour (LCW). Digestible energy (DE, MJ/kg of dry matter - DM) and digestible protein (DP, g/kg DM) values were obtained from regression analysis with three dietary incorporation levels of ELM or LCW: a basal diet (0%) and two diets with 8 or 16% of ELM or LCW, respectively. The regression curves were obtained from data collected in a faecal digestibility trial (n=10 rabbits per diet, 1:1 sex ratio, 42 to 46 d old) fed at two levels of inclusion (80 or 160 g/kg DM) of each ingredient in a basal diet formulated for growing rabbits (substitution method). The regression coefficients for DE (DE=10.4+0.003×ELW) and DP (DP=145.4+0.103×ELW) obtained for the ELM were significant (P<0.001). The estimated DE and DP values for the ELM were: 13.7±0.81 MJ/kg DM and 248.6±11.4 g/kg DM. The regression coefficient for DE (DE=10.4+0.011×LCW) for the LCW was significant (P<0.001), for an estimated DE content of 20.8±0.9 MJ/kg DM. The regression coefficient for DP (DP=145.6+0.011×LCW) for the LCW was not different from zero (P=0.09), giving an estimated DP of 166.9±11.3 g/kg DM. The ELM seems to be an adequate protein source for growing rabbits. The LCW appear to be an interesting composite ingredient. It provides moderate amounts of fibre fractions in addition to energy. Moreover, both ingredients contain high levels of C18:3 omega-3 fatty acids (ELM: 34.5 and LCW: 52.9 g/kg DM). These ingredients should be included up to 10% in feed formula. Based on their characteristics, these novel ingredients may improve the feed quality of growing rabbits.
References:
Agradi, S., Sulce, M., Menchetti, L., Vigo, D., Castrica, M., Barbato, O., Andoni, E., Quattrone, A., Munga, A., Marongiu, M.L., Curone, G., Brecchia, G. 2023. Dietary supplementation with n-3 polyunsaturated fatty acids: effects on reproductive and productive performance and meat quality in rabbit breeding. Animal Nutrition, 14, 70–78. https://doi.org/10.1016/j.aninu.2023.03.009
Alzueta, C., RodrÍguez, M.L., Cutuli, M.T., RebolÉ, A., Ortiz, L.T., Centeno, C., Treviño, J., 2003. Effect of whole and demucilaged linseed in broiler chicken diets on digesta viscosity, nutrient utilisation and intestinal microflora. British Poultry Science, 44, 67-74. https://doi.org/10.1080/0007166031000085337
Bekhit, A.E.-D.A., Shavandi, A., Jodjaja, T., Birch, J., Teh, S., Mohamed Ahmed, I.A., Al-Juhaimi, F.Y., Saeedi, P., Bekhit, A.A. 2018. Flaxseed: Composition, detoxification, utilization, and opportunities. Biocatalysis and Agricultural Biotechnology,13, 129-152. https://doi.org/10.1016/j.bcab.2017.11.017
Bianchi, M., Petracci, M., Cavani, C. 2006. Effects of dietary inclusion of dehydrated lucerne and whole linseed on rabbit meat quality. World Rabbit Science, 14, 247-258. https://doi.org/10.4995/wrs.2006.562
Bianchi, M., Petracci, M., Cavani, C. 2009. The influence of linseed on rabbit meat quality. World Rabbit Science, 17, 97-107. https://doi.org/10.4995/wrs.2009.663
Dalle Zotte, A., Cullere, M., Martins, C., Alves, S.P., Freire, J.P.B., Falcão-e-Cunha, L., Bessa, R.J.B. 2018. Incorporation of Black Soldier Fly (Hermetia illucens L.) larvae fat or extruded linseed in diets of growing rabbits and their effects on meat quality traits including detailed fatty acid composition. Meat Science, 146, 50-58. https://doi.org/10.1016/j.meatsci.2018.08.002
Delgado, R., Abad-Guamán, R., Nicodemus, N., Villamide, M.J., Ruiz-López, N., Carabaño, R., Menoyo, D., García, J. 2018. Effect of level of soluble fiber and n-6/n-3 fatty acid ratio on performance of rabbit does and their litters. Journal of Animal Science 96, 1084-1100. https://doi.org/10.1093/jas/sky019
Eiben, Cs., Végi, B., Virág, Gy., Gódor-Surmann, K., Kustos, K., Maró, A., Odermatt, M., Zsédely, E., Tóth, T., Schmidt, J., Fébel, H., 2011. Effect of level and source of vitamin E addition of a diet enriched with sunflower and linseed oils on growth and slaughter traits of rabbits. Livestock Science, 139, 196-205. https://doi.org/10.1016/j.livsci.2011.01.010
Eiben, Cs., Végi, B., Virág, Gy., Gódor-Surmann, K., Maró, A., x Odermatt, A., Zsédely, E., Tóth, T., Schmidt, J. 2010. Effect of different dietary ratios of sunflower and linseed oils on growth and carcass traits of rabbits. Livestock Science, 131, 15-22. https://doi.org/10.1016/j.livsci.2010.02.017.
European Group on Rabbit Nutrition (EGRAN). 2001. Technical note: attempts to harmonize chemical analyses of feeds and faeces, for rabbit feed evaluation. World Rabbit Science, 9, 57-64. https://doi.org/10.4995/wrs.2001.446
Hanif Palla, A., Gilani, A.-H., 2015. Dual effectiveness of flaxseed in constipation and diarrhea: possible mechanism. Journal of Ethnopharmacology, 169, 60–68. https://doi.org/10.1016/j.jep.2015.03.064
Imran, M., Ahmad, N., Anjum, F.M., Khan, M.K., Mushtaq, Z., Nadeem, M., Hussain, S., 2015. Potential protective properties of flax lignan secoisolariciresinol diglucoside. Nutrition Journal, 14, 71. https://doi.org/10.1186/s12937-015-0059-3
Jahanian, R., Rasouli, E. 2016. Effect of extrusion processing of soybean meal on ileal amino acid digestibility and growth performance of broiler chicks. Poultry Science, 95, 2871-2878. https://doi.org/10.3382/ps/pew178
Kouba, M., Benatmane, F., Blochet, J.E., Mourot, J. 2008. Effect of a linseed diet on lipid oxidation, fatty acid composition of muscle, perirenal fat, and raw and cooked rabbit meat. Meat Science, 80, 829-834. https://doi.org/10.1016/j.meatsci.2008.03.029
Matics, Zs., Cullere, M., Szín, M., Gerencsér, Zs., Szabó, A., Fébel, H., Odermatt, M., Radnai, I., Dalle Zotte, A., Szendrő, Zs. 2017. Effect of a dietary supplementation with linseed oil and selenium to growing rabbits on their productive performances, carcass traits and fresh and cooked meat quality. Journal of Animal Physiology and Animal Nutrition, 101, 685-693. https://doi.org/10.1111/jpn.12589
Mattioli, S., Bosco, A.D., Maranesi, M., Petrucci, L., Rebollar, P.G., Castellini, C., 2019. Dietary fish oil and flaxseed for rabbit does: fatty acids distribution and Δ6-desaturase enzyme expression of different tissues. Animal 13, 1934-1942. https://doi.org/10.1017/S175173111900020X
Menchetti, L., Canali, C., Castellini, C., Boiti, C., Brecchia, G. 2018. The different effects of linseed and fish oil supplemented diets on insulin sensitivity of rabbit does during pregnancy. Research in Veterinary Science, 118, 126-133. https://doi.org/10.1016/j.rvsc.2018.01.024
Pérez, J.M., Lebas, F., Gidenne, T., Maertens, L., Xiccato, G., Parigi-Bini, R., Dalle Zotte, A., Cossu, M.E, Carazzolo, A., Villamide, M.J., Carabaño, R., Fraga, M.J., Ramos, M.A., Cervera, C., Blas, E., Fernández, J., Falcão-e-Cunha, L., Bengala Freire J. 1995. European reference method for in vivo determination of diet digestibility in rabbits. World Rabbit Science, 3, 41-43. https://doi.org/10.4995/wrs.1995.239
Pirmohammadi, A., Khalaji, S., Yari, M. 2019. Effects of linseed expansion on its dietary molecular structures, and on broiler chicks digestive enzymes activity, serum metabolites, and Ileal morphology. Journal of Applied Poultry Research, 28, 997-1012. https://doi.org/10.3382/japr/pfz061
Treviño, J., Rodrı́guez, M.L., Ortiz, L.T., Rebolé, A., Alzueta, C., 2000. Protein quality of linseed for growing broiler chicks. Animal Feed Science and Technology, 84, 155-166. https://doi.org/10.1016/S0377-8401(00)00128-0
Van Soest, P.J., Wine, R.H. 1967. Use of detergents in the analysis of fibrous feeds. IV. Determination of plant cell-wall constituents, Journal of Association of Official Analytical Chemists, 50, 50-55. https://doi.org/10.1093/jaoac/50.1.50
Villamide M.J., García J., Cervera C., Blas E., Maertens L., Perez J.M. 2003. Comparison among methods of nutritional evaluation of dietary ingredients for rabbits. Animal Feed Science and Technology, 109, 195-207. https://doi.org/10.1016/S0377-8401(03)00177-9
Wu, M., Li, D., Wang, L.-J., Zhou, Y.-G., Brooks, M.S.-L., Chen, X.D., Mao, Z.-H. 2008. Extrusion detoxification technique on flaxseed by uniform design optimization. Separation and Purification Technology, 61, 51-59. https://doi.org/10.1016/j.seppur.2007.09.016
